This church was organized about 1710 and became a member of the first Charles Towne Presbytery established by the Rev. Archibald Stobo in 1722. The earliest pastor of whom there is any record was the Rev. Mr. Turnbull who served from 1728 to 1737. In 1793, a house of worship was built on Wadmalaw Island for the purpose of uniting that people with the people of Johns Island in the work of Christ. In 1909, the Wadmalaw church was organized as a separate unit and in 1925 the church on Johns Island changed its name back to the Johns Island Presbyterian Church.

The present sanctuary is believed to have been built in 1719. It was enlarged in 1823 and a gallery was added to the interior at that time for the seating of slaves.

The church building has survived the Revolutionary War, the Civil War and the earthquake of 1886. After hundreds of years, it is in a wonderful state of preservation. To the best of our knowledge, our sanctuary is older than any to be found of any denomination in the five major cities of Colonial America, namely Charleston, Williamsburg, New York, Philadelphia or Boston.

The building in which we now worship was erected 13 years before George Washington was born and 57 years before the signing of the Declaration of Independence.

A two-story Sunday School building was added to the back of the sanctuary in 1935 and another Educational and Fellowship building was erected in 1954. A complete restoration of the sanctuary was accomplished in 1992.

The church supported the first Presbyterian missionary to Africa, the Rev. Dr. John Wilson, who went there in 1833.

Since 1728 the congregation has had 28 pastors with the Rev. Jonathan Van Deventer presently serving in that capacity.

The church is on the National Register of Historic Places and is also an American Presbyterian and Reformed Historical Site.

Our history has been blessed by our Lord Christ and for that we are extremely grateful.